Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses
Identifieur interne : 002F59 ( Main/Exploration ); précédent : 002F58; suivant : 002F60Patterned two-photon illumination by spatiotemporal shaping of ultrashort pulses
Auteurs : Eirini Papagiakoumou [France] ; Vincent De Sars [France] ; Dan Oron [Israël] ; Valentina Emiliani [France]Source :
- Optics Express [ 1094-4087 ] ; 2008.
English descriptors
Abstract
Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanning-less, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.
Url:
DOI: 10.1364/OE.16.022039
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en"> <p>Multiphoton excitation by temporally focused pulses can be combined with spatial Fourier-transform pulse shaping techniques to enhance spatial control of the excitation volume. Here we propose and demonstrate an optical system for the generation of such spatiotemporally engineered light pulses using a combination of spatial control by a two-dimensional reconfigurable light modulator, with a dispersive optical setup for temporal focusing. We show that although the properties of a holographic beam significantly differ from those of plane-wave illumination used in previous temporal focusing realizations, this leads only to a slightly reduced axial resolution. We show that the system can provide scanning-less, arbitrarily shaped, depth resolved excitation patterns that offer new perspectives for multiphoton photoactivation and optical lithography applications.</p>
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